A4040-129 exam Dumps Source : Accessment: IBM i 7.1 Administration
Test Code : A4040-129
Test designation : Accessment: IBM i 7.1 Administration
Vendor designation : IBM
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Georgia's Metropolitan Atlanta swiftly Transit Authority uses IBM Watson IoT gear to uphold predict, identify and restore abilities issues with critical device and machinery
ARMONK, N.Y., Feb. 28, 2019 /PRNewswire/ -- IBM (NYSE: IBM) these days announced a brand original portfolio of web of issues (IoT) solutions that team synthetic intelligence (AI) and superior analytics to abet asset intensive corporations, such because the Metropolitan Atlanta quick Transit Authority (MARTA), to augment renovation concepts. The solution is designed to uphold companies to lessen expenses and in the reduction of the possibility of failure from actual property equivalent to motors, manufacturing robots, turbines, mining machine, elevators, and electrical transformers. IBM Maximo Asset efficiency management (APM) options assemble statistics from physical belongings in near precise-time and supply insights on existing operating conditions, foretell talents concerns, determine complications and tender restoration recommendations.
IBM)" alt="IBM these days introduced a original portfolio of web of things options, IBM Maximo Asset performance administration, bringing together synthetic intelligence and advanced analytics to assist asset intensive organizations, such as the Metropolitan Atlanta rapid Transit Authority (MARTA) in Georgia, to enhance preservation innovations. The solution collects records in proximate real-time from any physical asset, reminiscent of vehicles, manufacturing robots, mills, mining machine, elevators, electrical transformers and extra, an d provide insights on present working situations, predicts competencies considerations, identifies complications and presents restore techniques. (IBM)" />
businesses in asset-intensive industries like energy and utilities, chemical compounds, oil and gas, manufacturing, and transportation, can absorb thousands of belongings that are necessary to operations. These assets are more and more producing large quantities of information on their working circumstances. holding these assets up and running is essential to operations and monitoring and optimizing property on-going maintenance, fix, and substitute selections requires regular evaluation. in response to analyst solid Aberdeen research, unplanned downtime can can freight a corporation as much as $260,000 an hour.
company leaders can now recognize if a laptop is probably going to fail, the consume of advanced analytics and AI to capture into account timing, foretell consequences and establish issues. IBM's APM solutions uphold companies shift asset renovation thoughts from preventative to predictive and prescriptive with the aid of integrating disparate information sources to determine property in exigency of attention and recommending actions.
This retort enhances a corporation's current trade asset management (EAM) capabilities, reminiscent of IBM's market leading Maximo EAM solution, and integrates seamlessly with other EAM suppliers. It contains:
IBM will additionally present the APM suite customized for specific industries, genesis with APM for power and Utilities (E&U). This provides business-selected capabilities to research and act on insights from utility belongings and contains risk/criticality scoring, health and degradation fashions, common trade information mannequin, and weather facts integration.
"it's essential for businesses to assume about how helpful their renovation practices are. With the launch of its original Asset efficiency management solutions, IBM is assisting companies do perception-pushed selections with an integrated portfolio of superior analytics and AI capabilities to assist them enrich operational effectiveness and efficiency," pointed out Kareem Yusuf, Ph.D., universal manager, IBM Watson IoT. "Maximo APM is designed to enhance a firm's existing enterprise Asset administration gadget by artery of addressing every points of asset performance together with fitness, predictive maintenance and restore optimization."
MARTA keeps Atlanta, Georgia running
The Metropolitan Atlanta speedy Transit Authority (MARTA), the foremost public transit agency in the Atlanta metropolitan enviornment, is working with IBM to build in constrain a predictive preservation retort to enhance reliability of property, minimize expenses and create a Transit Asset management (TAM) device that offers asset inventory, situation assessment, efficiency measures and selection support. through data mining, computer researching and AI, MARTA can entry and dissect statistics to enhanced recall the circumstance of machine classified in the classes of life defense, operation critical and operation uphold to determine potential issues of a "gadget" with assorted stakeholders. subsequently, the retort will allow MARTA to seamlessly circulation from monitoring asset efficiency KPIs to predicting and fighting asset failures.
"MARTA is on course to circle into the primary North American public transit company to obtain ISO 55000 certification. taking fragment with IBM gives MARTA with the innovation from a expertise icon, which fortifies us as an industry chief in Transit Asset management," talked about Remy Saintil, director of facilities at MARTA.
About IBMIBM options are designed to allow customers to augment the operational efficiency of their physical belongings and reduce fees during the power of IoT information and synthetic intelligence. by using amassing and curating statistics from billions of related contraptions, sensors, and methods international, they empower companies to derive original insights from their equipment, automobiles and amenities. IBM's trade knowledge, leading protection, and options constructed for a hybrid cloud atmosphere firmly establish us as a leader in IoT. For extra assistance on IBM Watson IoT, delight talk over with www.ibm.com/iot.
IBM corporation emblem. (PRNewsfoto/IBM)greater
View usual content to down load multimedia:http://www.prnewswire.com/news-releases/ibm-brings-ai-and-advanced-analytics-to-the-industrial-world-300804229.html
February 18, 2019 Timothy Prickett Morgan
well, that took byalongshot a long time. After what seems like eons of nudging and cajoling and pushing, IBM is making the IBM i operating gear and its built-in database administration gadget, as well as the application construction gear and other programs application, attainable on its self-branded IBM Cloud public cloud.
massive Blue previewed its plans to bring each IBM i and AIX to the IBM Cloud at its annual suppose conference in Las Vegas, on scale out machines aimed toward little and medium corporations as well as to clients who want to hasten clusters of machines, and on scale up programs that absorb NUMA electronics that more tightly cluster them into shared reminiscence techniques.
There are a lot of questions about how this might breathe every breathe packaged up and bought under the unwieldy designation of the IBM vigour methods digital Server on IBM Cloud. however they are able to let you know every that they understand and fill you in as they gain information of greater.
The vim programs slices served up on the IBM Cloud operating IBM i and AIX absorb been first rolled out for early entry to chosen valued clientele to check the APIs for the wield plane, which they presume is in keeping with the PowerVC implementation of OpenStack. The prefatory customers who're kicking the tires had been supposed to simplest installation evolution and test environments or protype code, not at every the comfort working in production. This was absolutely supposed to breathe a proof of concept setup at this point, as you could behold from the rollout roadmap:
The alpha and beta trying out for the energy programs slices on the IBM Cloud is on a edifying looking quickly music, operating from February into March, as which you could see, and commonplace availability is slated for a while in March. doubtless somewhere towards the end, they would wager. IBM is at the genesis making the power puffs – what else are you able to designation them as shorthand? – obtainable in the early entry exams in its US East datacenter in the Washington DC metro area; it isn't clear what iron turned into used. The alpha and beta testing is being completed on a group of vigour S922 scale out machines, which can breathe in response to a pair of Power9 processors and which frankly is a large box for many IBM i stores every with the aid of itself, as well as on energy E880 scale up methods, that are being scaled as much as sixteen sockets and which consume the older Power8 processors. every of the machines consume ten-core processors, which are the sweet spot between efficiency, chip yield, and charge within the energy techniques line. The Power9 chips within the energy S922 servers hasten at 2.8 GHz and might turbo as much as three.9 GHz if there is satisfactory thermal headroom; these chips appear to breathe they may absorb four threads per core (often known as SMT4), which isn't as a total lot threading as the ordinary Power8 chip offers in scale out machines with SMT8 threading (eight threads per core) and twice as many cores per die (24 cores versus 12 cores). Anyway, it seems like the power S922 is being equipped with the heavy cores, now not the mild ones. The Power8 chips within the power E880 are operating at 4.19 GHz and additionally sheperd SMT8 threading (there was only a heavy core prerogative here); every sixteen cores are populated with ten-core Power8 chips, with a maximum scalability of 160 cores in a lone ratiocinative partition on the IBM Cloud.
each of the machines are being configured with 32 GB reminiscence sticks, which can breathe once again at the sweet spot between means, bandwidth, and charge per bit, and IBM is allowing valued clientele to configure the machines with anywhere from 8 GB to 64 GB of leading reminiscence per core. if you exigency denser reminiscence to bolster the skill, that you would breathe able to double it up the consume of sixty four GB reminiscence sticks, and then double it up again to 128 GB sticks, however this may breathe a custom order and it will can freight significantly extra, they consider. IBM is providing disk and glitter storage as much as 2 TB for autochthonous storage on the situations and growing to breathe at 10 GB increments. external storage for applications and databases is on Storwize V7000 disk arrays in the racks proximate this vigour iron, and are linked to the servers via Fibre Channel switches. valued clientele can share machines or employ dedicated iron, reckoning on their proclivities for absolute security and coping with stentorian neighbors on the digital infrastructure.
Steve Sibley vp and offering supervisor for the Cognitive programs division at IBM, gave me a sneak peek at the future energy cloud slices on a talk over with to the Austin, Texas labs final week, forward of the assume convention. here is how the facets and functions of the ratiocinative partitions on the IBM Cloud are shaping up:
Sibley told us that the pricing changed into now not yet set in stone for the digital energy capacity on the IBM Cloud, but when pressed about evaluating the cost between buying apparatus and renting it on the cloud, Sibley estimated that for a given IBM i or AIX workload running on in a similar artery configured hardware and ratiocinative partitions, running the cloud instance would freight about 30 p.c to 40 p.c extra on the illustration working full out over three years compared to buying the iron and the consume of it for three years. The difference, of direction, is that IBM is managing the vim systems and purchasing the datacenter, the electricity, the cooling, the security, patch updates on firmware, the installing of the PowerVM hypervisor and its replace, and the setting up of the IBM i or AIX working device. When organizations purchase their own energy systems machines, until they bag augmented features from IBM or a enterprise companion, it’s every on them. Pricing for the digital machines, the reminiscence, the storage, and the working gear and databases are every set per hour.
incidentally, it isn't clear what IBM i and AIX releases are supported on the machines, but most likely the existing IBM i 7.2 and IBM i 7.three releases are supported on the IBM Cloud as well as AIX 7.1 and AIX 7.2, that are furthermore present. Linux isn't mentioned as a supported platform, but SAP HANA workloads are and that means that there is a Linux – and above every red Hat enterprise Linux – beneath it considering that HANA in-reminiscence databases are not supported on any Unix variant, or IBM i for that remember.
by means of March, IBM plans to absorb the IBM i and AIX instances purchasable in creation in its US East (Washington DC) and US South (Dallas) regions, and in the 2d quarter IBM plans to roll it in its European datacenter in its eu imperative (Frankfurt) area, with different datacenters coming out as require pulls and IBM pushes.
we are able to capture a glance on the pricing as soon because it turns into obtainable and spot how it every stacks up, and bag the suggestions of alternative IBM i cloud avid gamers about what huge Blue is doing.
Public Cloud Dreaming For IBM i
Skytap Says It’s structure a ‘authentic Cloud’ offering for IBM i
a far better approach to skin The IBM i Cloud Cat
What Cloud providers should conclude To entice IBM i
Google near Launching IBM i Cloud service, Will Says
Is The ‘Golden Age’ of Computing Leaving IBM i at the back of?\
Ensono Emerges With IBM i Cloud providing
Clouds grow, however Can IBM i observe?
IBM ultimately receives Power8 Machines On SoftLayer Cloud
All signals point To The Cloud, however Will IBM i mob comply with?
mobile Apps And The IBM i concern manufacturing unit
Infor Readies IBM i Cloud
IBM i Finds a space in the Cloud
vigour programs Coming To The SoftLayer Cloud
IBM Brags About Its Cloud Prowess due to SoftLayer
IBM Buys SoftLayer To build Out hosting, Cloud agencies
Two putaquestionto TPM Questions: precise IBM i Clouds And Apple buying IBM i
IBM i 7.1 Tech Refresh activities live Partition Mobility
AWS/four hundred: Amazon Builds An AS/four hundred-oid Cloud
wanted: Cloud-i i-nfrastructure
The X factor: Head within the Clouds
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Feb 28, 2019 (Heraldkeeper via COMTEX) -- Global Airport Digitization Industry
New Study On "2018-2025 Airport Digitization Market Global Key Player, Demand, Growth, Opportunities and Analysis Forecast" Added to prudent Guy Reports Database
This report focuses on the global Airport Digitization status, future forecast, growth opportunity, key market and key players. The study objectives are to present the Airport Digitization evolution in United States, Europe and China.
In 2017, the global Airport Digitization market size was million US$ and it is expected to achieve million US$ by the discontinue of 2025, with a CAGR of during 2018-2025.
The key players covered in this studyCisco SystemsIBMMicrosoftSiemensSITAAppleScarabeeWind RiverDaifukuLiving PlanIT
Try Sample Report @https://www.wiseguyreports.com/sample-request/3552470-global-airport-digitization-market-size-status-and-forecast-2018-2025
Market segment by Type, the product can breathe split intoHardwareSoftwareService
Market segment by Application, split intoPassenger Screening and SecurityBaggage ServicesPassenger AssistanceOther
Market segment by Regions/Countries, this report coversUnited StatesEuropeChinaJapanSoutheast AsiaIndiaCentral & South America
The study objectives of this report are:To dissect global Airport Digitization status, future forecast, growth opportunity, key market and key players.To present the Airport Digitization evolution in United States, Europe and China.To strategically profile the key players and comprehensively dissect their evolution scheme and strategies.To define, record and forecast the market by product type, market and key regions.
For minute Reading delight visit WiseGuy Reports @ https://www.wiseguyreports.com/reports/3552470-global-airport-digitization-market-size-status-and-forecast-2018-2025
Some Major Points from Table of content:
1 Report Overview1.1 Study Scope1.2 Key Market Segments1.3 Players Covered1.4 Market Analysis by Type1.4.1 Global Airport Digitization Market Size Growth Rate by sort (2013-2025)1.4.2 Hardware1.4.3 Software1.4.4 Service1.5 Market by Application1.5.1 Global Airport Digitization Market share by Application (2013-2025)1.5.2 Passenger Screening and Security1.5.3 Baggage Services1.5.4 Passenger Assistance1.5.5 Other1.6 Study Objectives1.7 Years Considered
2 Global Growth Trends2.1 Airport Digitization Market Size2.2 Airport Digitization Growth Trends by Regions2.2.1 Airport Digitization Market Size by Regions (2013-2025)2.2.2 Airport Digitization Market share by Regions (2013-2018)2.3 Industry Trends2.3.1 Market Top Trends2.3.2 Market Drivers2.3.3 Market Opportunities
3 Market share by Key Players3.1 Airport Digitization Market Size by Manufacturers3.1.1 Global Airport Digitization Revenue by Manufacturers (2013-2018)3.1.2 Global Airport Digitization Revenue Market share by Manufacturers (2013-2018)3.1.3 Global Airport Digitization Market Concentration Ratio (CR5 and HHI)3.2 Airport Digitization Key Players Head office and district Served3.3 Key Players Airport Digitization Product/Solution/Service3.4 Date of Enter into Airport Digitization Market3.5 Mergers & Acquisitions, Expansion Plans
4 Breakdown Data by sort and Application4.1 Global Airport Digitization Market Size by sort (2013-2018)4.2 Global Airport Digitization Market Size by Application (2013-2018)
5 United States5.1 United States Airport Digitization Market Size (2013-2018)5.2 Airport Digitization Key Players in United States5.3 United States Airport Digitization Market Size by Type5.4 United States Airport Digitization Market Size by Application
6 Europe6.1 Europe Airport Digitization Market Size (2013-2018)6.2 Airport Digitization Key Players in Europe6.3 Europe Airport Digitization Market Size by Type6.4 Europe Airport Digitization Market Size by Application
7 China7.1 China Airport Digitization Market Size (2013-2018)7.2 Airport Digitization Key Players in China7.3 China Airport Digitization Market Size by Type7.4 China Airport Digitization Market Size by Application
8 Japan8.1 Japan Airport Digitization Market Size (2013-2018)8.2 Airport Digitization Key Players in Japan8.3 Japan Airport Digitization Market Size by Type8.4 Japan Airport Digitization Market Size by Application
9 Southeast Asia9.1 Southeast Asia Airport Digitization Market Size (2013-2018)9.2 Airport Digitization Key Players in Southeast Asia9.3 Southeast Asia Airport Digitization Market Size by Type9.4 Southeast Asia Airport Digitization Market Size by Application
10 India10.1 India Airport Digitization Market Size (2013-2018)10.2 Airport Digitization Key Players in India10.3 India Airport Digitization Market Size by Type10.4 India Airport Digitization Market Size by Application
11 Central & South America11.1 Central & South America Airport Digitization Market Size (2013-2018)11.2 Airport Digitization Key Players in Central & South America11.3 Central & South America Airport Digitization Market Size by Type11.4 Central & South America Airport Digitization Market Size by Application
12 International Players Profiles12.1 Cisco Systems12.1.1 Cisco Systems Company Details12.1.2 Company Description and trade Overview12.1.3 Airport Digitization Introduction12.1.4 Cisco Systems Revenue in Airport Digitization trade (2013-2018)12.1.5 Cisco Systems Recent Development12.2 IBM12.2.1 IBM Company Details12.2.2 Company Description and trade Overview12.2.3 Airport Digitization Introduction12.2.4 IBM Revenue in Airport Digitization trade (2013-2018)12.2.5 IBM Recent Development12.3 Microsoft12.3.1 Microsoft Company Details12.3.2 Company Description and trade Overview12.3.3 Airport Digitization Introduction12.3.4 Microsoft Revenue in Airport Digitization trade (2013-2018)12.3.5 Microsoft Recent Development12.4 Siemens12.4.1 Siemens Company Details12.4.2 Company Description and trade Overview12.4.3 Airport Digitization Introduction12.4.4 Siemens Revenue in Airport Digitization trade (2013-2018)12.4.5 Siemens Recent Development12.5 SITA12.5.1 SITA Company Details12.5.2 Company Description and trade Overview12.5.3 Airport Digitization Introduction12.5.4 SITA Revenue in Airport Digitization trade (2013-2018)12.5.5 SITA Recent Development12.6 Apple12.6.1 Apple Company Details12.6.2 Company Description and trade Overview12.6.3 Airport Digitization Introduction12.6.4 Apple Revenue in Airport Digitization trade (2013-2018)12.6.5 Apple Recent Development12.7 Scarabee12.7.1 Scarabee Company Details12.7.2 Company Description and trade Overview12.7.3 Airport Digitization Introduction12.7.4 Scarabee Revenue in Airport Digitization trade (2013-2018)12.7.5 Scarabee Recent Development12.8 Wind River12.8.1 Wind River Company Details12.8.2 Company Description and trade Overview12.8.3 Airport Digitization Introduction12.8.4 Wind River Revenue in Airport Digitization trade (2013-2018)12.8.5 Wind River Recent Development12.9 Daifuku12.9.1 Daifuku Company Details12.9.2 Company Description and trade Overview12.9.3 Airport Digitization Introduction12.9.4 Daifuku Revenue in Airport Digitization trade (2013-2018)12.9.5 Daifuku Recent Development12.10 alive PlanIT12.10.1 alive PlanIT Company Details12.10.2 Company Description and trade Overview12.10.3 Airport Digitization Introduction12.10.4 alive PlanIT Revenue in Airport Digitization trade (2013-2018)12.10.5 alive PlanIT Recent Development
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Figure 2 shows a schematic representation of the study design. Sixteen vigorous virile BALB/c mice were assigned to one of four radiopharmaceutical groups. Intraperitoneal (i.p.) administration of 15 mg/kg DOX was applied in every groups every three weeks for one, two, three, or four cycles, respectively. The maximum cumulative dose comprised 60 mg/kg given over 12 weeks, which corresponds to a potentially cardiotoxic dose of 1050 mg per cycle for a 70-kg virile patient10. At baseline and after completing each cycle of DOX, the cardiac and other organ biodistribution of the four radiopharmaceuticals was investigated approximately one hour after intravenous (i.v.) tail vein administration. After a group of four mice completed the regimen, the left ventricular (LV) duty was evaluated by transthoracic echocardiography. Then animals were sacrificed for non-imaging assessment, which included deoxynucleotidyl transferase biotin-dUTP nick-end labeling (TUNEL) staining, cardiac expression of Bcl-2, p53, caspase 3, caspase 8, and HIF-1α and Δψm by Western blot (WB) analysis, immunohistochemistry (IHC), and fluorescence-activated cell sorting (FACS)/flow cytometry. Furthermore, a group of four vigorous BALB/c mice per radiopharmaceutical served as controls, as they were treated at the same time-points and conditions with saline administration. They were furthermore imaged after every cycle, but were only sacrificed after completing every four saline cycles. The study was approved by the animal trust committee of the University of Pisa in accordance with germane animal trust guidelines.Figure 2
Schematic study design. Non-imaging markers are WB, IHC, and FACS expression markers. † = sacrifice of mice.Radiopharmaceutical preparation and administration 99mTc-sestamibi
The lipophilic cation Sestamibi (Cardiolite, Bristol-Myers Squibb) was radiolabeled by adding water to 1–3 ml of sodium pertechnetate (99mTcO4)Na and heated for ten minutes at 100 °C. After cooling, a monovalent lipophilic cationic intricate was retrieved (99mTc-sestamibi-6–2-metossi-isobutyl-isonitril), of which an mediocre of 18.5 MBq in 0.1 mL was injected i.v.99mTc-Annexin V
Human recombinant Annexin V (National Institutes of Health, Bethesda, MD, USA) was radiolabeled through combined in vitro incubation at latitude temperature for 30 minutes of 60–90 μg di-HYNIC-Annexin V diluted in 0.5 ml of physiological saline solution, 0.74–1.11 MBq pertechnetate anion (99mTcO4−), which was freshly eluted by a 6 GBq generator (Amersham, GE HealthCare), 10–20 μg of reduction agent (stannous chloride dehydrate, SnCl2−2H2O), and 1.5 mg of tricin (N-[tri(hydroxymethyl)methyl]glycine) as co-ligand. Labeling submit was 85–90%. 99mTc-Annexin V was then injected in a 0.1 mL solution containing on mediocre 18.5 MBq of 99mTc-Annexin V, which corresponds to a total protein concentration of 4.5 ng per animal.99mTc-glucaric acid
99mTc-glucaric acid (Molecular Targeting Technologies, Inc. West Chester, PA, USA) was prepared from a lyophilized kit containing 12.5 mg of D-glucaric acid and 0.15 mg of stannous chloride11 by adding 1480 MBq (40 mCi) freshly eluted 99mTc-pertechnetate as previously described12. Labeling efficiency (always >98%) was checked by ascending chromatography. The radiopharmaceutical was injected in 0.1 mL solution, containing on mediocre 18.5 MBq 99mTc-glucaric acid.[18F]FDG
The glucose analogue [18F]FDG was provided as a bulk vial (Gluscan, GE Healthcare), from which a sample of 18.5 MBq in 0.1 ml was injected. To avoid the intake of carbohydrates, the mice that received [18F]FDG were provided a fat-enriched diet.Echocardiographic evaluation
LV duty was evaluated with transthoracic echocardiography before the mice were euthanized, approximately 10 minutes after initiation of sedation. M-mode images were used for measurements of LV end-diastolic internal diameter (LVEDD) and LV end-systolic internal diameter (LVESD) in two consecutive beats. Fractional shortening (FS) was calculated as FS% = [(LVEDD − LVESD)/LVEDD] × 100.Biodistribution and histological analysis
After completion of their regimen and one hour after injection of the radiopharmaceuticals, animals were anesthetized and sacrificed. For biodistribution studies, samples of thyroid, kidney, tail, bone, lung, heart, muscle, blood, stomach, skin, liver, spleen, intestine, and urine were removed and weighed immediately. Radioactivity of these samples was registered for 180 seconds by a gamma counter (2470 Automatic Gamma Counter Wizard, PerkinElmer, Waltham, MA, USA), along with an aliquot of the injective and the tail to reform for partial extravasation, expressed as a percentage of the injected dose per gram of tissue (%ID/g).
Before gamma counting, two three-millimeter cardiac tissue slices were obtained for histology: one was frozen at −80 °C in dry ice and the other was placed in 4% formalin. After treatment with a protease inhibitor (1:100 in 10 mM Tris buffer, pH 8), apoptotic cells were assessed with direct fluorescence fragment discontinue labeling of DNA breaks (TUNEL kit, Fluorescein FragEL, Calbiochem, San Diego, CA, USA). Caspase 3 and HIF-1α were immunostained using antihuman-mouse active antibodies (rabbit anti-caspase 3 and rabbit anti-HIF polyclonal antibody, Chemicon International, Temecula, CA, USA) after incubation with a fluorescent-labeled secondary antibody (Alexa Fluor 488 goat anti-rabbit IGg, Thermo Fisher Scientific, Waltham, MA, USA). Cells stained with TUNEL, caspase 3, and HIF-1α were counted in ten randomly selected high-power tumor fields (magnification x200) and expressed as a percentage of total cells. Furthermore, cardiac samples were analyzed by Western blotting to identify the specific amount of Bcl-2, p53 (both Santa Cruz Biotechnology Inc., Dallas, TX, USA), caspase 3, and caspase 8 (both Cell Signaling Technologies Inc., Danvers, MA, USA).Measurement of Δψm
Inner Δψm was measured by a lone fluorescent lipophilic cation 5,5′,6,6′-tetrachloro- 1,1′,3,3′-tetraethylbenzimidazolocarbocyanine iodide (JC-1) that marks the loss of potential. For this purpose, mitochondria were isolated from myocardial tissue using a dedicated kit (Mytocondria Isolation Kit, Sigma-Aldrich, St. Louis, MO, USA) stained with JC-1 and analyzed by FACS (MACSQuant, Miltenyi Biotec, Bergisch Gladbach, Germany). JC-1, concentrated in the mitochondrial matrix of classic cells due to the electrochemical potential gradient, forms red fluorescent aggregates. Any event that dissipates the mitochondrial membrane potential prevents the accumulation of the JC-1 dye in the mitochondria. Accordingly, red stained cells depict intact mitochondria, whereas when the dye is dispersed throughout the entire cell, a shift from red (J-aggregates) to green fluorescence (JC-1 monomers) is observed. A red-to-green (i.e., intact to disrupt mitochondrial) ratio was performed and described as upper and lower border (UR and LR). Consequently, a decreasing ratio was considered a disintegration of membrane potential.Statistical analysis
Biodistribution and immunofluorescent data are expressed as value and median %ID/g tissue and standard divergence (SD). Data were analyzed by non-parametrical tests (Kruskal-Wallis and Dunn) to evaluate the differences in groups at different time points and by Mann-Whitney U to test the significance between DOX-treated and DOX-naïve mice. Furthermore, Spearman’s rho was used to assess correlations. Significance was set at p < 0.05. Statistics were performed using IBM SPSS 20 for Windows. The datasets generated and/or analyzed as fragment of the current study are available from the corresponding author by reasonable request.
ContractingGSA mulls recompete of SAM.gov contract
The universal Services Administration granted IBM a six-month $16 million extension on its condense to operate and modernize the SAM.gov system as the agency considers whether to build the trade up for bidding.
The extension was announced in a contracting document dated Aug. 13 that cited an urgent exigency to withhold IBM on the job while the agency explores the possibility of a competitive offering.
IBM has held the System for Award Management Architecture and Operations condense Services condense for eight years, and it is leading the trouble to modernize the system of systems that government agencies and vendors consume to manage hundreds of billions in government contracts annually.
IBM's condense expired Aug. 14. GSA had intended to award IBM another long-term deal, but according to contracting documents, that paddle was overturned because of a ruling that more market research needs to breathe conducted to uphold a decision to justify a sole source award.
The agency touted IBM's rating in a recent performance assessment.
"IBM's performance is solid and continues to improve over the course of this contract. They absorb moved from the satisfactory/good flush to the very good/excellent flush in many areas. Their processes absorb matured. Their information base continues to grow. IBM continues to confederate with the government to overcome challenges and displays stalwart commitment to program success," the document stated.
GSA is in the midst of a major modernization of the SAM ecosystem, which touches every aspect of civilian and defense contracting, from solicitation to tracking spending. The trouble has been in progress since 2010, and the behind pace of the project has generated some ire on Capitol Hill, most notably from Sen. Claire McCaskill (D-Mo.), who threatened to hold up confirmation of Denise Turner Roth to lead GSA in 2015 in order to obtain answers to questions about the cost and timing of the project.
In September 2017, GSA launched a beta site that will eventually encompass nine fully modernized systems, eliminating the exigency for standalone sites such as FedBizOpps, the Federal Procurement Data System and others. So far, just one legacy system -- the Catalog of Federal Domestic Assistance -- has been retired.
Once the modernized system moves from beta to production, GSA anticipates more opportunities, including multiple little trade awards to breathe made for the operation, maintenance and enhancement of the original system.
impress Rockwell is a senior staff writer at FCW, whose beat focuses on acquisition, the Department of Homeland Security and the Department of Energy.
Before joining FCW, Rockwell was Washington correspondent for Government Security News, where he covered every aspects of homeland security from IT to detection dogs and border security. Over the last 25 years in Washington as a reporter, editor and correspondent, he has covered an increasingly wide array of high-tech issues for publications like Communications Week, Internet Week, Fiber Optics News, tele.com magazine and Wireless Week.
Rockwell received a Jesse H. Neal Award for his toil covering telecommunications issues, and is a graduate of James Madison University.
Click here for previous articles by Rockwell. Contact him at email@example.com or supervene him on Twitter at @MRockwell4.
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